beta IV Tubulin (TUBB4A) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI6E3]

CAT#: TA503130AM

TUBB4 (beta IV Tubulin) mouse monoclonal antibody, clone OTI6E3 (formerly 6E3), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TUBB4 (RC203945, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TUBB4. Positive lysates LY401833 (100ug) and LC401833 (20ug) can be purchased separately from OriGene.

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  HEK293T cells transfected with either RC203945 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TUBB4 antibody (TA503130), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Jurkat cells, using anti-TUBB4 antibody (TA503130), (Red), compared to a nonspecific negative control antibody, (Blue).

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  Flow cytometric Analysis of Hela cells, using anti-TUBB4 antibody (TA503130), (Red), compared to a nonspecific negative control antibody, (Blue).

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  Western blot analysis of extracts (10ug) from 8 different cell lines by using anti-TUBB4 monoclonal antibody at 1:200 dilution.

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  Western blot analysis of extracts (10ug) from a mouse cell line and a mouse tissues by using anti-TUBB4 monoclonal antibody (1:200).

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Specifications

Product Data

• Clone Name: OTI6E3
• Applications: FC, WB
• Recommended Dilution: WB 1:2000, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TUBB4 (NP_006078) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 49.4 kDa
• Gene Name: tubulin beta 4A class IVa
• Database Link:
  NP_006078
  Entrez Gene 22153 MouseEntrez Gene 29213 RatEntrez Gene 10382 Human
  UniProt ID P04350
• Synonyms: beta-5; DYT4; TUBB4

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Gap junction, Pathogenic Escherichia coli infection