PHF21B Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI3D1]

CAT#: TA503177AM

PHF21B mouse monoclonal antibody, clone OTI3D1 (formerly 3D1), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PHF21B (RC204284, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PHF21B. Positive lysates LY408673 (100ug) and LC408673 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PHF21B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503177)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PHF21B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503177)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PHF21B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503177)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PHF21B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503177)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PHF21B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503177)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PHF21B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503177)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PHF21B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503177)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PHF21B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503177)

• Anti-PHF21B mouse monoclonal antibody (TA503177) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PHF21B (RC204284).

• HEK293T cells transfected with either RC204284 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PHF21B antibody (TA503177), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI3D1
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PHF21B(NP_612424) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 57.3 kDa
• Gene Name: PHD finger protein 21B
• Database Link:
  NP_612424
  Entrez Gene 112885 Human
  UniProt ID Q96EK2
• Synonyms: BHC80L; PHF4

Reference Data

• Protein Families: Druggable Genome, Transcription Factors