Aldehyde dehydrogenase 10 (ALDH3A2) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI2A7]

CAT#: TA503256AM

ALDH3A2 mouse monoclonal antibody, clone OTI2A7 (formerly 2A7), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALDH3A2 (RC200648, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALDH3A2. Positive lysates LY422196 (100ug) and LC422196 (20ug) can be purchased separately from OriGene.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and ALDH3A2-Knockout HeLa cells (KO, Cat# LC832790) were separated by SDS-PAGE and immunoblotted with anti-ALDH3A2 monoclonal antibody TA503256 (1:500`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ALDH3A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503256)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-ALDH3A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503256)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ALDH3A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503256)

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  HEK293T cells transfected with either RC200648 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ALDH3A2 antibody (TA503256), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI2A7
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150, FLOW 1:100
• Reactivities: Human, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ALDH3A2(NP_001026976) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 57.5 kDa
• Gene Name: aldehyde dehydrogenase 3 family member A2
• Database Link:
  NP_001026976
  Entrez Gene 65183 RatEntrez Gene 224 Human
  UniProt ID P51648
• Background: Aldehyde dehydrogenase isozymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. This gene product catalyzes the oxidation of long-chain aliphatic aldehydes to fatty acid. Mutations in the gene cause Sjogren-Larsson syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
• Synonyms: ALDH10; FALDH; SLS

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Arginine and proline metabolism, Ascorbate and aldarate metabolism, beta-Alanine metabolism, Butanoate metabolism, Fatty acid metabolism, Glycerolipid metabolism, Glycolysis / Gluconeogenesis, Histidine metabolism, Limonene and pinene degradation, Lysine degradation, Metabolic pathways, Propanoate metabolism, Pyruvate metabolism, Tryptophan metabolism, Valine, leucine and isoleucine degradation