TRAP alpha (SSR1) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI 5D5]

CAT#: TA503265BM

SSR1 (TRAP alpha) mouse monoclonal antibody, clone OTI 5D5 (formerly 5D5), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSR1 (RC202408, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSR1. Positive lysates LY401093 (100ug) and LC401093 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SSR1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and SSR1-Knockout HeLa cells (KO, Cat# LC812609) were separated by SDS-PAGE and immunoblotted with anti-SSR1 monoclonal antibody TA503265 (1:2000`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

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  HEK293T cells transfected with either RC202408 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SSR1 antibody (TA503265), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Hela cells, using anti-SSR1 antibody (TA503265), (Red), compared to a nonspecific negative control antibody, (Blue).

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  Flow cytometric Analysis of Jurkat cells, using anti-SSR1 antibody (TA503265), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI 5D5
• Applications: FC, WB
• Recommended Dilution: WB 1:500, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SSR1 (NP_003135) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 32.1 kDa
• Gene Name: signal sequence receptor subunit 1
• Database Link:
  NP_003135
  Entrez Gene 107513 MouseEntrez Gene 6745 Human
  UniProt ID P43307
• Background: The signal sequence receptor (SSR) is a glycosylated endoplasmic reticulum (ER) membrane receptor associated with protein translocation across the ER membrane. The SSR consists of 2 subunits, a 34-kD glycoprotein encoded by this gene and a 22-kD glycoprotein. This gene generates several mRNA species as a result of complex alternative polyadenylation. This gene is unusual in that it utilizes arrays of polyA signal sequences that are mostly non-canonical. [provided by RefSeq, Jul 2008]
• Synonyms: TRAPA

Reference Data

• Protein Families: Druggable Genome, Transmembrane