TFIIF (GTF2F1) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI4B10]

CAT#: TA503604BM

GTF2F1 mouse monoclonal antibody, clone OTI4B10 (formerly 4B10), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GTF2F1 (RC201294, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GTF2F1. Positive lysates LY400767 (100ug) and LC400767 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GTF2F1 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-GTF2F1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503604)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-GTF2F1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503604)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-GTF2F1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503604)

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  HEK293T cells transfected with either RC201294 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GTF2F1 antibody (TA503604), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI4B10
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GTF2F1(NP_002087) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 58.1 kDa
• Gene Name: general transcription factor IIF subunit 1
• Database Link:
  NP_002087
  Entrez Gene 98053 MouseEntrez Gene 316123 RatEntrez Gene 476731 DogEntrez Gene 699221 MonkeyEntrez Gene 2962 Human
  UniProt ID P35269
• Synonyms: BTF4; RAP74; TF2F1; TFIIF

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Basal transcription factors