Acetyl CoA synthetase (ACSS2) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI3H4]

CAT#: TA503609BM

ACSS2 (Acetyl CoA synthetase) mouse monoclonal antibody, clone OTI3H4 (formerly 3H4), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACSS2 (RC204260, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACSS2. Positive lysates LY412981 (100ug) and LC412981 (20ug) can be purchased separately from OriGene.

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  HEK293T cells transfected with either RC204260 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ACSS2 antibody (TA503609), and then analyzed by flow cytometry.

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ACSS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503609)

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ACSS2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

Specifications

Product Data

• Clone Name: OTI3H4
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ACSS2(NP_061147) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 78.4 kDa
• Gene Name: acyl-CoA synthetase short chain family member 2
• Database Link:
  NP_061147
  Entrez Gene 60525 MouseEntrez Gene 311569 RatEntrez Gene 55902 Human
  UniProt ID Q9NR19
• Background: This gene encodes a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. The protein acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. Alternative splicing results in multiple transcript variants. [provided by RefSeq]
• Synonyms: ACAS2; ACECS; ACS; ACSA; dJ1161H23.1

Reference Data

• Protein Pathways: Glycolysis / Gluconeogenesis, Metabolic pathways, Propanoate metabolism, Pyruvate metabolism