Lipoprotein lipase (LPL) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI3C8]

CAT#: TA503785AM

LPL (Lipoprotein lipase) mouse monoclonal antibody, clone OTI3C8 (formerly 3C8), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LPL (RC203766, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LPL. Positive lysates LY400089 (100ug) and LC400089 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (10ug) from a mouse cell line and 2 different mouse tissues by using anti-LPL monoclonal antibody (1:200).

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-LPL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503785)

Specifications

Product Data

• Clone Name: OTI3C8
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids LPL(NP_000228) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: lipoprotein lipase
• Database Link:
  NP_000228
  Entrez Gene 16956 MouseEntrez Gene 24539 RatEntrez Gene 4023 Human
  UniProt ID P06858
• Background: LPL encodes lipoprotein lipase, which is expressed in heart, muscle, and adipose tissue. LPL functions as a homodimer, and has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. Severe mutations that cause LPL deficiency result in type I hyperlipoproteinemia, while less extreme mutations in LPL are linked to many disorders of lipoprotein metabolism. [provided by RefSeq]
• Synonyms: HDLCQ11; LIPD

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Glycerolipid metabolism, PPAR signaling pathway