CENPH Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI2A11]

CAT#: TA503912BM

CENPH mouse monoclonal antibody, clone OTI2A11 (formerly 2A11), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CENPH (RC204531, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CENPH. Positive lysates LY402956 (100ug) and LC402956 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CENPH monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Anti-CENPH mouse monoclonal antibody (TA503912) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CENPH (RC204531).

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  HEK293T cells transfected with either RC204531 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CENPH antibody (TA503912), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI2A11
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CENPH(NP_075060) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 28.3 kDa
• Gene Name: centromere protein H
• Database Link:
  NP_075060
  Entrez Gene 64946 Human
  UniProt ID Q9H3R5
• Background: Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex. [provided by RefSeq, Jul 2008]
• Synonyms: centromere protein H; homolog; kinetochore protein CENP-H; MIND kinetochore complex component; NNF1; PMF1

Reference Data

• Protein Families: Druggable Genome