NMT2 Mouse Monoclonal Antibody [Clone ID: OTI2A5]

CAT#: TA504177

NMT2 mouse monoclonal antibody, clone OTI2A5 (formerly 2A5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NMT2 (RC202876, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NMT2. Positive lysates LY417742 (100ug) and LC417742 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NMT2 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-NMT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504177)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-NMT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504177)

Specifications

Product Data

• Clone Name: OTI2A5
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human NMT2(NP_004799) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.79 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 56.8 kDa
• Gene Name: N-myristoyltransferase 2
• Database Link:
  NP_004799
  Entrez Gene 18108 MouseEntrez Gene 291318 RatEntrez Gene 9397 Human
  UniProt ID O60551
• Background: N-myristoyltransferase (NMT) catalyzes the reaction of N-terminal myristoylation of many signaling proteins. It transfers myristic acid from myristoyl coenzyme A to the amino group of a protein's N-terminal glycine residue. Biochemical evidence indicates the presence of several distinct NMTs, varying in apparent molecular weight and /or subcellular distribution. The predicted 498-amino acid of human NMT2 protein shares 77% and 96% sequence identity with human NMT1 and mouse Nmt2 comprise two distinct families of N-myristoyltransferases. [provided by RefSeq]

Reference Data

• Protein Families: Druggable Genome