Calpastatin (CAST) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1E7]

CAT#: TA504351BM

CAST (Calpastatin) mouse monoclonal antibody, clone OTI1E7 (formerly 1E7), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CAST (RC211776, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CAST. Positive lysates LY400663 (100ug) and LC400663 (20ug) can be purchased separately from OriGene.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and CAST-Knockout Hela cells (KO, Cat# LC810027) were separated by SDS-PAGE and immunoblotted with anti-CAST monoclonal antibody TA504351, (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-CAST mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504351)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-CAST mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504351)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-CAST mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504351)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CAST mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504351)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CAST mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504351)

• HEK293T cells transfected with either RC211776 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CAST antibody (TA504351), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI1E7
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CAST(NP_001741) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 84.8 kDa
• Gene Name: calpastatin
• Database Link:
  NP_001741
  Entrez Gene 831 Human
  UniProt ID P20810
• Background: The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq]
• Synonyms: BS-17; PLACK