CNOT4 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI3D11]

CAT#: TA504447BM

CNOT4 mouse monoclonal antibody, clone OTI3D11 (formerly 3D11), HRP conjugated

Product Images

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CNOT4 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Flow cytometric Analysis of Hela cells, using anti-CNOT4 antibody (TA504447), (Red), compared to a nonspecific negative control antibody, (Blue).

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  Flow cytometric Analysis of Jurkat cells, using anti-CNOT4 antibody (TA504447), (Red), compared to a nonspecific negative control antibody, (Blue).

Specifications

Product Data

• Clone Name: OTI3D11
• Applications: FC, WB
• Recommended Dilution: WB 1:2000, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to ammino acids 190-455 of human CNOT4(NP_037448) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 70.2 kDa
• Gene Name: CCR4-NOT transcription complex subunit 4
• Database Link:
  NP_037448
  Entrez Gene 53621 MouseEntrez Gene 312227 RatEntrez Gene 4850 Human
  UniProt ID O95628
• Background: The protein encoded by this gene is a subunit of the CCR4-NOT complex, a global transcriptional regulator. The encoded protein interacts with CNOT1 and has E3 ubiquitin ligase activity. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]
• Synonyms: CLONE243; NOT4; NOT4H

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: RNA degradation