PDLIM5 Mouse Monoclonal Antibody [Clone ID: OTI1A11]

CAT#: TA504449

PDLIM5 mouse monoclonal antibody, clone OTI1A11 (formerly 1A11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDLIM5 (RC200592, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDLIM5. Positive lysates LY416641 (100ug) and LC416641 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-PDLIM5 monoclonal antibody (1:200).

• 

  HEK293T cells transfected with either RC200592 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PDLIM5 antibody (TA504449), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI1A11
• Applications: FC, WB
• Recommended Dilution: WB 1:2000, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PDLIM5(NP_006448) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 3.37 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 63.8 kDa
• Gene Name: PDZ and LIM domain 5
• Database Link:
  NP_006448
  Entrez Gene 56376 MouseEntrez Gene 64353 RatEntrez Gene 10611 Human
  UniProt ID Q96HC4
• Background: The protein encoded by this gene is a LIM domain protein. LIM domains are cysteine-rich double zinc fingers composed of 50 to 60 amino acids that are involved in protein-protein interactions. LIM domain-containing proteins are scaffolds for the formation of multiprotein complexes. The proteins are involved in cytoskeleton organization, cell lineage specification, organ development, and oncogenesis. The encoded protein is also a member of the Enigma class of proteins, a family of proteins that possess a 100-amino acid PDZ domain in the N terminus and 1 to 3 LIM domains in the C terminus. Multiple transcript variants encoding different isoforms have been found for this gene, although not all of them have been fully characterized. [provided by RefSeq]
• Synonyms: ENH; ENH1; L9; LIM

Reference Data

• Protein Families: Druggable Genome