Mannose Phosphate Isomerase (MPI) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI1C7]

CAT#: TA504749AM

MPI mouse monoclonal antibody,clone 1C7, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MPI (RC208134, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MPI. Positive lysates LY419324 (100ug) and LC419324 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504749)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504749)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504749)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504749)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504749)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-MPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504749)

• Anti-MPI mouse monoclonal antibody (TA504749) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MPI (RC208134).

• HEK293T cells transfected with either RC208134 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MPI antibody (TA504749), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI1C7
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MPI(NP_002426) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 46.5 kDa
• Gene Name: mannose phosphate isomerase
• Database Link:
  NP_002426
  Entrez Gene 300741 RatEntrez Gene 4351 Human
  UniProt ID P34949
• Background: Phosphomannose isomerase catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate and plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib. [provided by RefSeq]
• Synonyms: CDG1B; PMI; PMI1

Reference Data

• Protein Families: ES Cell Differentiation/IPS
• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Fructose and mannose metabolism, Metabolic pathways