MAFB Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI3F6]

CAT#: TA505014AM

MAFB mouse monoclonal antibody,clone 3F6, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAFB (RC207311, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAFB. Positive lysates LY401676 (100ug) and LC401676 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MAFB monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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  HEK293T cells transfected with either RC207311 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAFB antibody (TA505014), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI3F6
• Applications: FC, WB
• Recommended Dilution: WB 1:2000, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 32-320 of human MAFB (NP_005452) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 35.6 kDa
• Gene Name: MAF bZIP transcription factor B
• Database Link:
  NP_005452
  Entrez Gene 16658 MouseEntrez Gene 54264 RatEntrez Gene 9935 Human
  UniProt ID Q9Y5Q3
• Background: The protein encoded by this gene is a basic leucine zipper (bZIP) transcription factor that plays an important role in the regulation of lineage-specific hematopoiesis. The encoded nuclear protein represses ETS1-mediated transcription of erythroid-specific genes in myeloid cells. This gene contains no introns. [provided by RefSeq]
• Synonyms: DURS3; KRML; MCTO

Reference Data

• Protein Families: Druggable Genome, Transcription Factors