CHN 1 (CHN1) Mouse Monoclonal Antibody [Clone ID: OTI1C9]

CAT#: TA505072S

CHN1 mouse monoclonal antibody, clone OTI1C9 (formerly 1C9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHN1 (RC222163, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHN1. Positive lysates LY400688 (100ug) and LC400688 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CHN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505072)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CHN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505072)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CHN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505072)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CHN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505072)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CHN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505072)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CHN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505072)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CHN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505072)

• HEK293T cells transfected with either RC222163 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CHN1 antibody (TA505072), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI1C9
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CHN1(NP_001813) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 53 kDa
• Gene Name: chimerin 1
• Database Link:
  NP_001813
  Entrez Gene 84030 RatEntrez Gene 108699 MouseEntrez Gene 1123 Human
  UniProt ID P15882
• Background: This gene encodes GTPase-activating protein for ras-related p21-rac and a phorbol ester receptor. It is predominantly expressed in neurons, and plays an important role in neuronal signal-transduction mechanisms. Mutations in this gene are associated with Duane's retraction syndrome 2 (DURS2). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]
• Synonyms: ARHGAP2; CHN; DURS2; NC; RHOGAP2