RHOJ Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1A9]

CAT#: TA505468BM

RHOJ mouse monoclonal antibody,clone 1A9, HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RHOJ (RC209395, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RHOJ. Positive lysates LY412401 (100ug) and LC412401 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RHOJ mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505468)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RHOJ mouse monoclonal antibody. (TA505468)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-RHOJ mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505468)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-RHOJ mouse monoclonal antibody. (TA505468)

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-RHOJ mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505468)

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Specifications

Product Data

• Clone Name: OTI1A9
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human RHOJ(NP_065714) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 23.6 kDa
• Gene Name: ras homolog family member J
• Database Link:
  NP_065714
  Entrez Gene 80837 MouseEntrez Gene 299145 RatEntrez Gene 57381 Human
  UniProt ID Q9H4E5
• Background: This gene encodes one of the many small GTP-binding proteins in the Rho family shown to be associated with focal adhesions in endothelial cells (PMID: 21148427, 22103495). The encoded protein is activated by vascular endothelial growth factor and may regulate angiogenesis. [provided by RefSeq, Dec 2011]
• Synonyms: ARHJ; RASL7B; TC10B; TCL

Reference Data

• Protein Families: Druggable Genome