BID Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI3C8]

CAT#: TA506768AM

BID mouse monoclonal antibody, clone OTI3C8 (formerly 3C8), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BID (RC207261, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BID. Positive lysates LY420074 (100ug) and LC420074 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BID monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-BID mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506768)

• 

  Anti-BID mouse monoclonal antibody (TA506768) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BID (RC207261).

Specifications

Product Data

• Clone Name: OTI3C8
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:400~4000, IHC 1:150, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human BID(NP_001187) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: BH3 interacting domain death agonist
• Database Link:
  NP_001187
  Entrez Gene 637 Human
  UniProt ID P55957
• Background: This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008]
• Synonyms: FP497

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Natural killer cell mediated cytotoxicity, p53 signaling pathway, Pathways in cancer, Viral myocarditis