Osteopontin (SPP1) Rabbit Polyclonal Antibody

CAT#: TA590059

Rabbit Polyclonal Osteopontin Antibody

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPP1 (RC210592, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPP1. Positive lysates LY400422 (100ug) and LC400422 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SPP1 Rabbit Polyclonal antibody. (TA590059)

• 

  Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-SPP1 Rabbit Polyclonal antibody. (TA590059)

• 

  Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-SPP1 Rabbit Polyclonal antibody. (TA590059)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB 1:5000~20000, IHC 1:150, ELISA 1:100-1:2000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: DNA immunization. This antibody was made against a protein fragment from the C Terminus Region
• Formulation: 20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
• Concentration: 1.01mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: secreted phosphoprotein 1
• Database Link:
  NP_001035149
  Entrez Gene 6696 Human
  UniProt ID P10451
• Background: Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine.Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin. Osteopontin is overexpressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma.
• Synonyms: BNSP; BSPI; ETA-1; OPN
• Note: This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: ECM-receptor interaction, Focal adhesion, Toll-like receptor signaling pathway