PKC zeta (PRKCZ) Rabbit Polyclonal Antibody

CAT#: TA590141

Rabbit Polyclonal PKC zeta Antibody

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKCZ (RC202472, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKCZ. Positive lysates LY400967 (100ug) and LC400967 (20ug) can be purchased separately from OriGene.

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  ELISA: PKC zeta Antibody

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: WB 1:20000,ELISA 1:100-1:2000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: DNA immunization. This antibody is specific for the Middle Region of the target protein.
• Formulation: 20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
• Concentration: 1.11679 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: protein kinase C zeta
• Database Link:
  NP_002735
  Entrez Gene 5590 Human
  UniProt ID Q05513
• Background: Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Synonyms: PKC-ZETA; PKC2
• Note: This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Chemokine signaling pathway, Endocytosis, Insulin signaling pathway, Tight junction, Type II diabetes mellitus