OLIG2 Rabbit Polyclonal Antibody

CAT#: TA590593

Rabbit Polyclonal OLIG2 Antibody

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OLIG2 (RC208209, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OLIG2. Positive lysates LY417055 (100ug) and LC417055 (20ug) can be purchased separately from OriGene.

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  Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11; Lane 2: RT-4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: TonsilThis validation was performed by Protein Atlas and the presentation of data is for informational purposes only.

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  Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in a subset of glial cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.

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  Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleoli & golgi apparatus.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:20000, ELISA 1:100-1:2000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: DNA immunization. This antibody was made against a protein fragment from the N Terminus Region
• Formulation: 20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
• Concentration: 0.9mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: oligodendrocyte transcription factor 2
• Database Link:
  NP_005797
  Entrez Gene 10215 Human
  UniProt ID Q13516
• Background: Olig2 (oligodendrocyte transcription factor 2) is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. It cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. It is an antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development.
• Synonyms: BHLHB1; bHLHe19; OLIGO2; PRKCBP2; RACK17
• Note: This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

• Protein Families: Druggable Genome, Transcription Factors