PTP1B (PTPN1) Rabbit Polyclonal Antibody

CAT#: TA590672

Rabbit Polyclonal PTPN1 Antibody

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTPN1 (RC204902, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPN1. Positive lysates LY419087 (100ug) and LC419087 (20ug) can be purchased separately from OriGene.

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  Western Blot: - Samples: Lane 1, Marker: 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2, RT-4 Lane 3, U-251MG sp Lane 4, Human Plasma Lane 5, Liver Lane 6, Tonsil, Target weight 50 kDa.

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  Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-PTPN1 Rabbit Polyclonal antibody. (TA590672)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-PTPN1 Rabbit Polyclonal antibody. (TA590672)

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-PTPN1 Rabbit Polyclonal antibody. (TA590672)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PTPN1 Rabbit Polyclonal antibody. (TA590672)

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB 1:20000, IHC 1:150,ELISA 1:100-1:2000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: DNA immunization. In vivo generated recombinant protein fragment
• Formulation: 20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
• Concentration: 0.8mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: protein tyrosine phosphatase non-receptor type 1
• Database Link:
  NP_002818
  Entrez Gene 5770 Human
  UniProt ID P18031
• Background: The non-transmembrane protein tyrosine phosphatase, PTP1B, comprises 435 amino acids, of which the C-terminal 114 residues have been implicated in controlling both localization and function of this enzyme. Increased phosphorylation of PTP1B is seen to accompany the transition from G2 to M phase of the cell cycle. Immunofluorescence experiments indicate that PTP-1B is localized predominantly in the endoplasmic reticulum (ER). Subcellular fractionation is consistent with this localization and establishes that PTP-1B is tightly associated with microsomal membranes, with its phosphatase domain oriented towards the cytoplasm. The finding of a tyrosine phosphatase on the ER suggests new possibilities for cellular events controlled by tyrosine phosphorylation (2). It has also been shown that PTP1B is a novel substrate for Akt and that phosphorylation of PTP1B by Akt at Ser(50) may negatively modulate its phosphatase activity creating a positive feedback mechanism for insulin signaling (3).
• Synonyms: PTP1B
• Note: This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

• Protein Families: Druggable Genome, Phosphatase, Transmembrane
• Protein Pathways: Adherens junction, Insulin signaling pathway