Ki67 (MKI67) Rat Monoclonal Antibody [Clone ID: OTI3B6]

CAT#: TA801343

Ki67 rat monoclonal antibody, clone OTI3B6 (formerly 3B6)

Product Images

Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and MKI67-Knockout Hela cells (KO, Cat# LC810145) were separated by SDS-PAGE and immunoblotted with anti-MKI67 monoclonal antibody TA801343. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control (1:200).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MKI67 rat monoclonal antibody. (TA801343; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MKI67 rat monoclonal antibody. (TA801343; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MKI67 rat monoclonal antibody. (TA801343; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MKI67 rat monoclonal antibody. (TA801343; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MKI67 rat monoclonal antibody. (TA801343; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MKI67 rat monoclonal antibody. (TA801343; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-MKI67 rat monoclonal antibody. (TA801343; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MKI67 rat monoclonal antibody. (TA801343; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-MKI67 rat monoclonal antibody. (TA801343; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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Specifications

Product Data

• Clone Name: OTI3B6
• Applications: IHC, WB
• Recommended Dilution: IHC 1:150
• Reactivities: Human
• Host: Rat
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1160-1493 of human MKI67 (NP_002408) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: marker of proliferation Ki-67
• Database Link:
  NP_002408
  Entrez Gene 4288 Human
  UniProt ID P46013
• Background: This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]
• Synonyms: KIA; MIB-; MIB-1; PPP1R105

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS