p23 (PTGES3) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI2D2]

CAT#: TA803433AM

PTGES3 mouse monoclonal antibody,clone 2D2, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTGES3 (RC201254, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTGES3. Positive lysates LY401975 (100ug) and LC401975 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of A549 cell lysate (35ug) by using anti-PTGES3 monoclonal antibody. Dilution: 1:500

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and PTGES3-Knockout 293T cells (KO, Cat# LC811134) were separated by SDS-PAGE and immunoblotted with anti-PTGES3 monoclonal antibody TA803433, (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PTGES3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA803433)

Specifications

Product Data

• Clone Name: OTI2D2
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PTGES3 (NP_006592) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 18.5 kDa
• Gene Name: prostaglandin E synthase 3
• Database Link:
  NP_006592
  Entrez Gene 56351 MouseEntrez Gene 362809 RatEntrez Gene 10728 Human
  UniProt ID Q15185
• Synonyms: cPGES; P23; TEBP

Reference Data

• Protein Families: Druggable Genome, Nuclear Hormone Receptor