Laminin 5 (LAMB3) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI3A2]

CAT#: TA804424BM

LAMB3 mouse monoclonal antibody, clone OTI3A2 (formerly 3A2), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LAMB3 (RC215510, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LAMB3. Positive lysates LY424856 (100ug) and LC424856 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LAMB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804424)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-LAMB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804424)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-LAMB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804424)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-LAMB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804424)

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Specifications

Product Data

• Clone Name: OTI3A2
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 424-773 of human LAMB3 (NP_000219) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 127.6 kDa
• Gene Name: laminin subunit beta 3
• Database Link:
  NP_000219
  Entrez Gene 3914 Human
  UniProt ID Q13751
• Synonyms: AI1A; BM600-125KDA; LAM5; LAMNB1

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: ECM-receptor interaction, Focal adhesion, Pathways in cancer, Small cell lung cancer