MLH1 Mouse Monoclonal Antibody [Clone ID: OTI1C1]

CAT#: TA805066

MLH1 mouse monoclonal antibody, clone OTI1C1 (formerly 1C1)

Product Images

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MLH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 3min, TA805066) (1:300)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human Esophagus using anti-MLH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 3min, TA805066) (1:300)

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  Immunohistochemical staining of paraffin-embedded Human Gastric Carcinoma using anti-MLH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 3min, TA805066) (1:300)

Specifications

Product Data

• Clone Name: OTI1C1
• Applications: IHC
• Recommended Dilution: IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 299-527 of human MLH1(NP_000240) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 84.4 kDa
• Gene Name: mutL homolog 1
• Database Link:
  NP_000240
  Entrez Gene 17350 MouseEntrez Gene 81685 RatEntrez Gene 4292 Human
  UniProt ID P40692
• Background: This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+phenotype) found in HNPCC. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described, but their full-length natures have not been determined. [provided by RefSeq, Nov 2009]
• Synonyms: COCA2; FCC2; hMLH1; HNPCC; HNPCC2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Colorectal cancer, Endometrial cancer, Mismatch repair, Pathways in cancer