Peripherin (PRPH) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI2D5]

CAT#: TA806182BM

PRPH mouse monoclonal antibody, clone OTI2D5 (formerly 2D5), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRPH (RC207561, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRPH. Positive lysates LY401885 (100ug) and LC401885 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PRPH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806182)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PRPH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806182)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PRPH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806182)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PRPH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806182)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PRPH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806182)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PRPH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806182)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PRPH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806182)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PRPH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806182)

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Specifications

Product Data

• Clone Name: OTI2D5
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PRPH (NP_006253) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 53.5 kDa
• Gene Name: peripherin
• Database Link:
  NP_006253
  Entrez Gene 19132 MouseEntrez Gene 24688 RatEntrez Gene 5630 Human
  UniProt ID P41219
• Background: This gene encodes a cytoskeletal protein found in neurons of the peripheral nervous system. The encoded protein is a type III intermediate filament protein with homology to other cytoskeletal proteins such as desmin, and is a different protein that the peripherin found in photoreceptors. Mutations in this gene have been associated with susceptibility to amyotrophic lateral sclerosis. [provided by RefSeq, Jul 2008]
• Synonyms: NEF4; PRPH1

Reference Data

• Protein Pathways: Amyotrophic lateral sclerosis (ALS)