Retinoic Acid Receptor alpha (RARA) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI2C3]

CAT#: TA806484AM

RARA mouse monoclonal antibody, clone OTI2C3 (formerly 2C3), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RARA (RC200430, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RARA. Positive lysates LY400350 (100ug) and LC400350 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RARA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806484)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-RARA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806484)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-RARA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806484)

Specifications

Product Data

• Clone Name: OTI2C3
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 52-299 of human RARA(NP_000955) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 50.6 kDa
• Gene Name: retinoic acid receptor alpha
• Database Link:
  NP_000955
  Entrez Gene 19401 MouseEntrez Gene 24705 RatEntrez Gene 5914 Human
  UniProt ID P10276
• Background: This gene represents a nuclear retinoic acid receptor. The encoded protein, retinoic acid receptor alpha, regulates transcription in a ligand-dependent manner. This gene has been implicated in regulation of development, differentiation, apoptosis, granulopoeisis, and transcription of clock genes. Translocations between this locus and several other loci have been associated with acute promyelocytic leukemia. Alternatively spliced transcript variants have been found for this locus. [provided by RefSeq, Sep 2010]
• Synonyms: NR1B1; RAR

Reference Data

• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Pathways in cancer