5 Lipoxygenase (ALOX5) Mouse Monoclonal Antibody [Clone ID: OTI1C1]

CAT#: TA806887

ALOX5 mouse monoclonal antibody,clone OTI1C1

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALOX5 (RC217259, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALOX5 (1:2000). Positive lysates LY400234 (100ug) and LC400234 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from K562 cell line by using anti-ALOX5 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-ALOX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA806887) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue tissue using anti-ALOX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA806887) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-ALOX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA806887) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ALOX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA806887) (1:2000)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-ALOX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA806887) (1:2000)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-ALOX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA806887) (1:2000)

• Immunohistochemical staining of paraffin-embedded Human spleen tissue within the normal limits using anti-ALOX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA (1:2000)

• Immunohistochemical staining of paraffin-embedded Human appendix tissue within the normal limits using anti-ALOX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA (1:2000)

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Specifications

Product Data

• Clone Name: OTI1C1
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 18-364 of human ALOX5 (NP_000689) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 77.8 kDa
• Gene Name: arachidonate 5-lipoxygenase
• Database Link:
  NP_000689
  Entrez Gene 11689 MouseEntrez Gene 25290 RatEntrez Gene 240 Human
  UniProt ID P09917
• Synonyms: 5-LO; 5-LOX; 5LPG; LOG5

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Arachidonic acid metabolism, Metabolic pathways