Lymphocyte Activation Gene 3 (LAG3) Mouse Monoclonal Antibody [Clone ID: OTI3C6]

CAT#: TA807081

LAG3 mouse monoclonal antibody,clone OTI3C6

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LAG3 (RC220269, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LAG3 (1:500).

• 

  Western blot analysis of extracts (35ug) from 2 cell lines (Human PBMC treated with 10ug/ml PHA for 72h (Right)/untreated (Left)) lysates by using anti-LAG3 monoclonal antibody (1:150).

• 

  Flow cytometric analysis of living 293T cells transfected with LAG3 overexpression plasmid (RC220269), Red)/empty vector (PS100001, Blue) using anti-LAG3 antibody (TA807081). Cells incubated with a non-specific antibody (Green) were used as isotype control.

Specifications

Product Data

• Clone Name: OTI3C6
• Applications: FC, WB
• Recommended Dilution: WB 1:150~500, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 66-332 of human LAG3(NP_002277) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 57.45 kDa
• Gene Name: lymphocyte activating 3
• Database Link:
  NP_002277
  Entrez Gene 3902 Human
  UniProt ID P18627
• Background: Involved in lymphocyte activation. Binds to HLA class-II antigens. [UniProtKB/Swiss-Prot Function]
• Synonyms: CD223

Reference Data

• Protein Families: Transmembrane