SDCCAG10 (CWC27) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI4C1]

CAT#: TA808676BM

CWC27 mouse monoclonal antibody, clone OTI4C1 (formerly 4C1), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CWC27 (RC223566, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CWC27 (1:2000). Positive lysates LY417011 (100ug) and LC417011 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-CWC27 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808676) (1:150)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-CWC27 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808676) (1:150)

Specifications

Product Data

• Clone Name: OTI4C1
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 145-472 of human CWC27(NP_005860) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 53.7 kDa
• Gene Name: CWC27 spliceosome associated protein homolog
• Database Link:
  NP_005860
  Entrez Gene 10283 Human
  UniProt ID Q6UX04
• Background: PPIases accelerate the folding of proteins. [UniProtKB/Swiss-Prot Function]
• Synonyms: NY-CO-10; SDCCAG10