GATA3 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI10G7]

CAT#: TA809219BM

GATA3 mouse monoclonal antibody, clone OTI10G7, HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GATA3 (RC211904, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GATA3 (1:2000). Positive lysates LY424146 (100ug) and LC424146 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 2 different cell by using anti-GATA3 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-GATA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809219) (1:150)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-GATA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809219) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-GATA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809219) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-GATA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809219) (1:150)

• Immunocytochemistry staining of MCF-7 cells using anti-GATA3 mouse monoclonal antibody (TA809219) (Left). The right is negative control (1:5000).

• Immunocytochemistry staining of MDA-MB-468 cells using anti-GATA3 mouse monoclonal antibody (TA809219) (Left). The right is negative control (1:5000).

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Specifications

Product Data

• Clone Name: OTI10G7
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 155-443 of human GATA3 (NP_002042) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 47.9 kDa
• Gene Name: GATA binding protein 3
• Database Link:
  NP_001002295
  Entrez Gene 14462 MouseEntrez Gene 85471 RatEntrez Gene 2625 Human
  UniProt ID P23771
• Background: This gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in this gene are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia. [provided by RefSeq, Nov 2009]
• Synonyms: HDR; HDRS

Reference Data

• Protein Families: Adult stem cells, ES Cell Differentiation/IPS, Stem cell relevant signaling - JAK/STAT signaling pathway, Transcription Factors