TRMT12 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI1C9]

CAT#: TA809532AM

TRMT12 mouse monoclonal antibody,clone OTI1C9, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRMT12 (RC203982, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRMT12 (1:2000). Positive lysates LY402634 (100ug) and LC402634 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-TRMT12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809532) (1:500)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TRMT12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809532) (1:500)

Specifications

Product Data

• Clone Name: OTI1C9
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombiant protein of human TRMT12 (NP_060426) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 50.1 kDa
• Gene Name: tRNA methyltransferase 12 homolog
• Database Link:
  NP_060426
  Entrez Gene 55039 Human
  UniProt ID Q53H54
• Background: Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TRMT12 is the human homolog of a yeast gene essential for yW synthesis (Noma and Suzuki, 2006). [supplied by OMIM, Mar 2008]. COMPLETENESS: complete on the 3' end.
• Synonyms: TRM12; TYW2