METTL7A Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI4B10]

CAT#: TA809878AM

METTL7A mouse monoclonal antibody,clone OTI4B10, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY METTL7A (RC202601, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-METTL7A (1:2000). Positive lysates LY415471 (100ug) and LC415471 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-METTL7A monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-METTL7A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809878) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-METTL7A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809878) (1:2000)

Specifications

Product Data

• Clone Name: OTI4B10
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:2000
• Reactivities: Human, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 34-104 of human METTL7A(NP_054752) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 28.1 kDa
• Gene Name: methyltransferase like 7A
• Database Link:
  NP_054752
  Entrez Gene 315306 RatEntrez Gene 25840 Human
  UniProt ID Q9H8H3
• Background: Self-associates. [UniProtKB/Swiss-Prot Function]
• Synonyms: AAM-B

Reference Data

• Protein Families: Druggable Genome, Transmembrane