NEDD4 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI7A1]

CAT#: TA812647BM

NEDD4 mouse monoclonal antibody,clone OTI7A1, HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEDD4 (RC212738, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEDD4 (1:500). Positive lysates LY405051 (100ug) and LC405051 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI7A1
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 160-460 of human NEDD4 (NP_940682) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 140.7 kDa
• Gene Name: neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase
• Database Link:
  NP_940682
  Entrez Gene 4734 Human
  UniProt ID P46934
• Background: This gene is the founding member of the NEDD4 family of HECT ubiquitin ligases that function in the ubiquitin proteasome system of protein degradation. The encoded protein contains an N-terminal calcium and phospholipid binding C2 domain followed by multiple tryptophan-rich WW domains and, a C-terminal HECT ubiquitin ligase catalytic domain. It plays critical role in the regulation of a number of membrane receptors, endocytic machinery components and the tumor suppressor PTEN. [provided by RefSeq, Jul 2016]
• Synonyms: NEDD4-1; RPF1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis, Ubiquitin mediated proteolysis