CD137 (TNFRSF9) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI4G1]

CAT#: TA813073AM

TNFRSF9 mouse monoclonal antibody,clone OTI4G1, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNFRSF9 (RC200664, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNFRSF9 (1:500).

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  Flow cytometric analysis of living 293T cells transfected with TNFRSF9 overexpression plasmid (RC200664), Red)/empty vector (PS100001, Blue) using anti-TNFRSF9 antibody (TA813073). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

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  Flow cytometric analysis of living PBMCs treated with 10ug/ml PHA for 72h (Right)/untreated (Left) using anti-TNFRSF9 antibody (TA813073) (1:100).

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  Flow cytometric analysis of living PBMCs treated with 10ug/ml PHA for 72h (Right) using anti-TNFRSF9 antibody (TA813073). Cells incubated with a non-specific antibody (Left) were used as isotype control (1:100).

Specifications

Product Data

• Clone Name: OTI4G1
• Applications: FC, WB
• Recommended Dilution: WB 1:500, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TNFRSF9 (NP_001552) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: tumor necrosis factor receptor superfamily member 9
• Database Link:
  NP_001552
  Entrez Gene 3604 Human
  UniProt ID Q07011
• Background: Receptor for TNFSF9/4-1BBL. Possibly active during T cell activation. [UniProtKB/Swiss-Prot Function]
• Synonyms: 4-1BB; CD137; CDw137; ILA

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction