DDB1 Rabbit Polyclonal Antibody

CAT#: TA890041S

Rabbit polyclonal anti-DDB1 Antibody

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDB1 (RC208372, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDB1 rab. Positive lysates LY419658 (100ug) and LC419658 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from different cell lines or tissues by using anti-DDB1 rabbit polyclonal antibody (TA890041).

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500~1:1000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human DDB1
• Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: 1.62mg/ml
• Purification: Purified from the immunized serum by affinity chromatography (Protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 126.8 kDa
• Gene Name: damage specific DNA binding protein 1
• Database Link:
  NP_001914
  Entrez Gene 13194 MouseEntrez Gene 64470 RatEntrez Gene 1642 Human
  UniProt ID Q16531
• Background: The protein encoded by this gene is the large subunit (p127) of the heterodimeric DNA damage-binding (DDB) complex while another protein (p48) forms the small subunit. This protein complex functions in nucleotide-excision repair and binds to DNA following UV damage. Defective activity of this complex causes the repair defect in patients with xeroderma pigmentosum complementation group E (XPE) - an autosomal recessive disorder characterized by photosensitivity and early onset of carcinomas. However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients. The protein encoded by this gene also functions as an adaptor molecule for the cullin 4 (CUL4) ubiquitin E3 ligase complex by facilitating the binding of substrates to this complex and the ubiquitination of proteins. [provided by RefSeq, May 2012]
• Synonyms: DDBA; UV-DDB1; XAP1; XPCE; XPE; XPE-BF

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Nucleotide excision repair, Ubiquitin mediated proteolysis