PSMA (FOLH1) Mouse Monoclonal Antibody [Clone ID: UMAB27]

CAT#: UM500027CF

Carrier-free (BSA/glycerol-free) FOLH1 mouse monoclonal antibody,clone UMAB27

Product Images

Western blot of cell lysates (35ug) from MDCK. Diluation: 1:500

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  Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen ). Diluation: 1:500.

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  Western blot of mouse tissue lysates (20ug) from Brain. Diluation: 1:500.

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  Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-FOLH1mouse monoclonal antibody. (Clone UMAB27, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-FOLH1mouse monoclonal antibody. (Clone UMAB27, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-FOLH1mouse monoclonal antibody. (Clone UMAB27, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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Specifications

Product Data

• Clone Name: UMAB27
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human FOLH1(NP_004467) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 84.2 kDa
• Gene Name: Homo sapiens folate hydrolase 1 (FOLH1), transcript variant 1, mRNA.
• Database Link:
  NP_004467
  Entrez Gene 2346 Human
  UniProt ID Q04609
• Background: This gene encodes a type II transmembrane glycoprotein belonging to the M28 peptidase family. The protein acts as a glutamate carboxypeptidase on different alternative substrates, including the nutrient folate and the neuropeptide N-acetyl-l-aspartyl-l-glutamate and is expressed in a number of tissues such as prostate, central and peripheral nervous system and kidney. A mutation in this gene may be associated with impaired intestinal absorption of dietary folates, resulting in low blood folate levels and consequent hyperhomocysteinemia. Expression of this protein in the brain may be involved in a number of pathological conditions associated with glutamate excitotoxicity. In the prostate the protein is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer. This gene likely arose from a duplication event of a nearby chromosomal region. Alternative splicing gives rise to multiple transcript variants encoding several different isoforms. [provided by RefSeq]
• Synonyms: FGCP; FOLH; GCP2; GCPII; mGCP; NAALAD1; NAALAdase; PSM; PSMA

Reference Data

• Protein Families: Druggable Genome, Protease, Transmembrane