Syntenin (SDCBP) Mouse Monoclonal Antibody [Clone ID: UMAB69]

CAT#: UM500057CF

Carrier-free (BSA/glycerol-free) SDCBP mouse monoclonal antibody,clone UMAB69

Product Images

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SDCBP monoclonal antibody (Clone UMAB69).

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  Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-SDCBP monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-SDCBP mouse monoclonal antibody. (UM500057; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SDCBP mouse monoclonal antibody. (UM500057; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded human prostate cancer using anti-SDCBP clone UMAB69 mouse monoclonal antibody (UM500057) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer using pressure chamber for 3 minutes at 110C. Membrane and cytoplasmic staining is seen in tumor cells.

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  Immunohistochemical staining of paraffin-embedded composit of 3 human liver cancer cases using anti-SDCBP clone UMAB69 mouse monoclonal antibody (UM500057) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer using pressure chamber for 3 minutes at 110C. Membrane and cytoplasmic staining is seen in tumor cells with various intensity.

• Immunohistochemical staining of paraffin-embedded composit of 3 human endometrial cancer cases using anti-SDCBP clone UMAB69 mouse monoclonal antibody (UM500057) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer using pressure chamber for 3 minutes at 110C. Membrane and cytoplasmic staining is seen in tumor cells with various intensity.

• Immunohistochemical staining of paraffin-embedded composit of 3 human ovarian cancer cases using anti-SDCBP clone UMAB69 mouse monoclonal antibody (UM500057) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer using pressure chamber for 3 minutes at 110C. Membrane and cytoplasmic staining is seen in tumor cells with various intensity.

• Immunohistochemical staining of paraffin-embedded composit of 2 human spleen cases using anti-SDCBP clone UMAB69 mouse monoclonal antibody (UM500057) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer using pressure chamber for 3 minutes at 110C. Membrane and cytoplasmic staining is seen in the cells of red pulp and white pulp.

• Immunohistochemical staining of paraffin-embedded tonsil using anti-SDCBP clone UMAB69 mouse monoclonal antibody (UM500057) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer using pressure chamber for 3 minutes at 110C. Membrane and cytoplasmic staining is seen in germinal and non germinal center of the the tonsil.

• Immunohistochemical staining of paraffin-embedded composit of 2 human melanoma cases using anti-SDCBP clone UMAB69 mouse monoclonal antibody (UM500057) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer using pressure chamber for 3 minutes at 110C. Membrane, nuclear, and cytoplasmic staining is seen in tumor cells with various intensity.

• Immunohistochemical staining of paraffin-embedded human brain using anti-SDCBP clone UMAB69 mouse monoclonal antibody (UM500057) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer using pressure chamber for 3 minutes at 110C. Weak membrane and cytoplasmic staining.

• Immunofluorescent staining of MCF-7 cells using anti-SDCBP mouse monoclonal antibody (UM500057, green, 1:50). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue).

• OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-SDCBP mouse monoclonal antibody (UM500057). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

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Specifications

Product Data

• Clone Name: UMAB69
• Applications: 10k-ChIP, IF, IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SDCBP(NP_005616) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 32.3 kDa
• Gene Name: Homo sapiens syndecan binding protein (SDCBP), transcript variant 1, mRNA.
• Database Link:
  NP_005616
  Entrez Gene 53378 MouseEntrez Gene 83841 RatEntrez Gene 6386 Human
  UniProt ID O00560
• Background: The protein encoded by this gene was initially identified as a molecule linking syndecan-mediated signaling to the cytoskeleton. The syntenin protein contains tandemly repeated PDZ domains that bind the cytoplasmic, C-terminal domains of a variety of transmembrane proteins. This protein may also affect cytoskeletal-membrane organization, cell adhesion, protein trafficking, and the activation of transcription factors. The protein is primarily localized to membrane-associated adherens junctions and focal adhesions but is also found at the endoplasmic reticulum and nucleus. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
• Synonyms: MDA-9; MDA9; ST1; SYCL; TACIP18

Reference Data

• Protein Families: Druggable Genome, Transmembrane