ZNF447 (ZSCAN18) Mouse Monoclonal Antibody [Clone ID: UMAB113]

CAT#: UM500080CF

Carrier-free (BSA/glycerol-free) ZSCAN18 mouse monoclonal antibody,clone UMAB113

Product Images

Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Thyroid, 7: Colon, 8: Spleen 9: Liver, 10: Ovary). Diluation: 1:500.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ZSCAN18 mouse monoclonal antibody. (UM500080; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-ZSCAN18 mouse monoclonal antibody. (UM500080; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-ZSCAN18 mouse monoclonal antibody. (UM500080; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-ZSCAN18 mouse monoclonal antibody. (UM500080; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human tonsil using anti-ZSCAN18 mouse monoclonal antibody. (UM500080; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunofluorescent staining of MCF-7 cells using anti-ZSCAN18 mouse monoclonal antibody (UM500080, green, 1:100). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue).

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Specifications

Product Data

• Clone Name: UMAB113
• Applications: IF, IHC, WB
• Recommended Dilution: IHC 1:200
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ZSCAN18(NP_076415) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 54.6 kDa
• Gene Name: Homo sapiens zinc finger and SCAN domain containing 18 (ZSCAN18), transcript variant 3, mRNA.
• Database Link:
  NP_076415
  Entrez Gene 65982 Human
  UniProt ID Q8TBC5
• Synonyms: ZNF447

Reference Data

• Protein Families: Transcription Factors