BBOX1 Mouse Monoclonal Antibody [Clone ID: UMAB120]

CAT#: UM500085

BBOX1 mouse monoclonal antibody,clone UMAB120

Product Images

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-BBOX1 mouse monoclonal antibody. (UM500085; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-BBOX1 mouse monoclonal antibody. (UM500085; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549, 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7). Diluation: 1:250

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  Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Uterus, 3: Omentum, 4: Breast, 5: Brain, 6: Thyroid, 7: Colon, 8: Spleen 9: Liver, 10: Ovary). Diluation: 1:500.

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  Immunohistochemical staining of paraffin-embedded human liver using anti-BBOX1 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM500085 requires HIER with citrate pH6.0 at 110°C for 3min using pressure chamber/cooker. The image shows strong cytoplasmic and membrane in the hepatocytes

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  Immunohistochemical staining of paraffin-embedded human kidney carcinoma using anti-BBOX1 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM500085 requires HIER with citrate pH6.0 at 110°C for 3min using pressure chamber/cooker. The composit image shows strong cytoplasmic, membrane, and nuclear in panel A tumor cells and no staining in panel B tumor cells but strong staining is normal tubule epithelial cells.

• Immunofluorescent staining of HeLa cells using anti-BBOX1 mouse monoclonal antibody (UM500085, green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue).

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Specifications

Product Data

• Clone Name: UMAB120
• Applications: IF, IHC, WB
• Recommended Dilution: IHC 1:100~200
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human BBOX1 (NP_003977) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5~1.0 mg/ml (Lot Dependent)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 44.5 kDa
• Gene Name: Homo sapiens gamma-butyrobetaine hydroxylase 1 (BBOX1), mRNA.
• Database Link:
  NP_003977
  Entrez Gene 170442 MouseEntrez Gene 8424 Human
  UniProt ID O75936
• Background: This gene encodes gamma butyrobetaine hydroxylase which catalyzes the formation of L-carnitine from gamma-butyrobetaine, the last step in the L-carnitine biosynthetic pathway. Carnitine is essential for the transport of activated fatty acids across the mitochondrial membrane during mitochondrial beta-oxidation. [provided by RefSeq, Jul 2008]
• Synonyms: BBH; BBOX; G-BBH; gamma-BBH

Reference Data

• Protein Pathways: Lysine degradation