CA12 Mouse Monoclonal Antibody [Clone ID: UMAB121]

CAT#: UM500086

CA12 mouse monoclonal antibody,clone UMAB121

Product Images

Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-CA12 mouse monoclonal antibody. (UM500086; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-CA12 mouse monoclonal antibody. (UM500086; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CA12 mouse monoclonal antibody. (UM500086; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Western blot of cell lysates (35ug) from A549. Diluation: 1:500

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-CA12 mouse monoclonal antibody. (UM500086; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunofluorescent staining of HeLa cells using anti-CA12 mouse monoclonal antibody (UM500086, green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20µm.

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Specifications

Product Data

• Clone Name: UMAB121
• Applications: IF, IHC, WB
• Recommended Dilution: IHC 1:100~200
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CA12(NP_001209) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5~1.0 mg/ml (Lot Dependent)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 36.8 kDa
• Gene Name: Homo sapiens carbonic anhydrase 12 (CA12), transcript variant 1, mRNA.
• Database Link:
  NP_001209
  Entrez Gene 771 Human
  UniProt ID O43570
• Background: Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Two transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
• Synonyms: CAXII; HsT18816

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Nitrogen metabolism