FTCD Mouse Monoclonal Antibody [Clone ID: UMAB123]

CAT#: UM500088CF

Carrier-free (BSA/glycerol-free) FTCD mouse monoclonal antibody,clone UMAB123

Product Images

Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549, 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7). Diluation: 1:500

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  Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen ). Diluation: 1:500.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-FTCD mouse monoclonal antibody. (UM500088; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-FTCD mouse monoclonal antibody. (UM500088; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-FTCD mouse monoclonal antibody. (UM500088; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-FTCD mouse monoclonal antibody. (UM500088; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunofluorescent staining of HeLa cells using anti-FTCD mouse monoclonal antibody (UM500088, green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20µm.

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Specifications

Product Data

• Clone Name: UMAB123
• Applications: IF, IHC, WB
• Recommended Dilution: IHC 1:100~200
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human FTCD(NP_006648) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 58.7 kDa
• Gene Name: Homo sapiens formimidoyltransferase cyclodeaminase (FTCD), transcript variant B, mRNA.
• Database Link:
  NP_006648
  Entrez Gene 10841 Human
  UniProt ID O95954
• Background: The protein encoded by this gene is a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool. Mutations in this gene are associated with glutamate formiminotransferase deficiency. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2009]
• Synonyms: LCHC1

Reference Data

• Protein Pathways: Histidine metabolism, Metabolic pathways, One carbon pool by folate