MSI1 Mouse Monoclonal Antibody [Clone ID: UMAB127]

CAT#: UM500092CF

Carrier-free (BSA/glycerol-free) MSI1 mouse monoclonal antibody,clone UMAB127

Product Images

Immunohistochemical staining of paraffin-embedded Human adult brain tissue using anti-MSI1 mouse monoclonal antibody. (UM500092; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human embryonic brain cortex tissue using anti-MSI1 mouse monoclonal antibody. (UM500092; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-MSI1 mouse monoclonal antibody. (UM500092; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded human colon using anti-MSI1 clone UMAB127 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. UM500092 requires heat-induced epitope retrieval with Accel pH 8.7 in a presure cooker for 3 minutes at 110C.

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  Immunohistochemical staining of paraffin-embedded human pancreas using anti-MSI1 clone UMAB127 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. UM500092 requires heat-induced epitope retrieval with Accel pH 8.7 in a presure cooker for 3 minutes at 110C. Cytoplasmic and membrane staining seen weakly on the glandular cells and strongly on the Islet of Langerhans

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  OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-MSI1 mouse monoclonal antibody (UM500092). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

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Specifications

Product Data

• Clone Name: UMAB127
• Applications: 10k-ChIP, IHC
• Recommended Dilution: IHC 1:100~200
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MSI1(NP_002433) produced in HEk293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 38.9 kDa
• Gene Name: Homo sapiens musashi RNA binding protein 1 (MSI1), mRNA.
• Database Link:
  NP_002433
  Entrez Gene 17690 MouseEntrez Gene 259272 RatEntrez Gene 4440 Human
  UniProt ID O43347
• Background: This gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Expression of this gene has been correlated with the grade of the malignancy and proliferative activity in gliomas and melanomas. A pseudogene for this gene is located on chromosome 11q13. [provided by RefSeq, Jul 2008]
• Synonyms: musashi 1; musashi homolog 1 (Drosophila)