EPCAM Mouse Monoclonal Antibody [Clone ID: UMAB132]

CAT#: UM500097CF

Carrier-free (BSA/glycerol-free) EPCAM mouse monoclonal antibody,clone UMAB132

Product Images

Western blot analysis of LOVO cell lysate (35ug) by using anti-EPCAM monoclonal antibody.

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  Western blot analysis of COLO205 cell lysate (35ug) by using anti-EPCAM monoclonal antibody.

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  Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-EPCAM monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

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  Western blot analysis of HEK293 cell lysate (35ug) by using anti-EPCAM monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-EPCAM mouse monoclonal antibody. (UM500097; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-EPCAM mouse monoclonal antibody. (UM500097; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-EPCAM mouse monoclonal antibody. (UM500097; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-EPCAMmouse monoclonal antibody. (UM500097; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-EPCAMmouse monoclonal antibody. (UM500097; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-EPCAM mouse monoclonal antibody. (UM500097; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-EPCAMEPCAMmonoclonal antibody. (UM500097; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-EPCAM mouse monoclonal antibody (UM500097). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

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Specifications

Product Data

• Clone Name: UMAB132
• Applications: 10k-ChIP, IHC, WB
• Recommended Dilution: WB 1:4000, IHC 1:100~200
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human EPCAM(NP_002345) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens epithelial cell adhesion molecule (EPCAM), mRNA.
• Database Link:
  NP_002345
  Entrez Gene 4072 Human
  UniProt ID P16422
• Background: This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]
• Synonyms: 4; DIAR5; EGP-2; EGP40; EGP314; ESA; HNPCC8; KS1; KSA; M4S1; MIC18; MK-1; TACSTD1; TROP1

Reference Data

• Protein Families: ES Cell Differentiation/IPS, Transmembrane