RTRAF Mouse Monoclonal Antibody [Clone ID: UMAB175]

CAT#: UM800068

C14orf166 mouse monoclonal antibody,clone UMAB175

Product Images

Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: Jurkat, 6: MDCK, 7: COS7, 8: PC-12, 9: MCF7).

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  Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen ). Diluation: 1:500.

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-C14orf166 mouse monoclonal antibody. (UM800068; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human tonsil using anti-C14orf166 mouse monoclonal antibody. (UM800068; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human skin tissue using anti-C14orf166 mouse monoclonal antibody. (UM800068; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-C14orf166 mouse monoclonal antibody. (UM800068; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

• Immunofluorescent staining of HeLa cells using anti-C14orf166 mouse monoclonal antibody (UM800068, green, 1:100). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue).

• OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-C14orf166 mouse monoclonal antibody (UM800068). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

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Specifications

Product Data

• Clone Name: UMAB175
• Applications: 10k-ChIP, IF, IHC, WB
• Recommended Dilution: IHC 1:100~200
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human C14orf166 (NP_057123) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5~1.0 mg/ml (Lot Dependent)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 27.9 kDa
• Gene Name: Homo sapiens RNA transcription, translation and transport factor (RTRAF), mRNA.
• Database Link:
  NP_057123
  Entrez Gene 51637 Human
  UniProt ID Q9Y224
• Synonyms: CGI-99; CGI99; CLE; CLE7; hCLE1; LCRP369; RLLM1