EGFR Mouse Monoclonal Antibody [Clone ID: UMAB233]

CAT#: UM800125CF

Carrier-free (BSA/glycerol-free) EGFR L858R mouse monoclonal antibody,clone UMAB233

Product Images

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue (Sample1 and sample3 are EGFR L858R positive NSCLC, sample2 and sample4 are EGFR L858R negative NSCLC) using anti-EGFR L858R Mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800125) (1:300)

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  Immunohistochemical staining of paraffin-embedded ERBB superfamily members cell pellets (Sample 1 is ERBB1 (RC217384), Sample 2 is ERBB2 (RC212583), Sample 3 is ERBB3 (RC209954), Sample 4 is ERBB4 (RC220449)) using anti-EGFR L858R Mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800125) (1:300)

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  Immunohistochemical staining of paraffin-embedded over-expressed wild type and mutant EGFR cell pellets (Sample 1 is EGFR (E746-A750 del), Sample 2 is EGFR (L858R), Sample 3 is 293T, Sample 4 is EGFR Wild Type) using anti-EGFR L858R Mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800125) (1:300)

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  Immunocytochemistry staining of cell lines (On the left is A549, on the right is NCI-H1975) using anti-EGFR L858R mouse monoclonal antibody (UM800125) (1:50).

Specifications

Product Data

• Clone Name: UMAB233
• Applications: IF, IHC
• Recommended Dilution: IHC 1:300, IF 1:50
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide around the L858R mutation region of the human EGFR conjugated to KLH
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 1, mRNA.
• Database Link:
  NP_005219
  Entrez Gene 13649 MouseEntrez Gene 24329 RatEntrez Gene 1956 Human
  UniProt ID P00533
• Synonyms: ERBB; ERBB1; HER1; mENA; NISBD2; PIG61

Reference Data

• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase, Secreted Protein, Stem cell relevant signaling - JAK/STAT signaling pathway, Transmembrane
• Protein Pathways: Adherens junction, Bladder cancer, Calcium signaling pathway, Colorectal cancer, Cytokine-cytokine receptor interaction, Dorso-ventral axis formation, Endocytosis, Endometrial cancer, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, MAPK signaling pathway, Melanoma, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton