Human IgM (heavy chain) Mouse Monoclonal Antibody [Clone ID: SPM557]

CAT#: AM50119PU-T

Human IgM (heavy chain) mouse monoclonal antibody, clone SPM557, Purified

Product Images

Formalin-Fixed, Paraffin-Embedded Human Tonsil stained with Human IgM Antibody Cat.-No AM50102PU-N (Clone SPM557).

Specifications

Product Data

• Clone Name: SPM557
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use Antibody without BSA for Coating.
  Flow Cytometry: 0.5-1 μg/10⁶ cells.
  Immunofluorescence: 0.5-1 μg/ml.
  Western Blot: 0.5-1 μg/ml.
  Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
  Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
  Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant heavy chain of human IgM.
• Specificity: Recognizes a protein of 75kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin’s lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
  Cellular Localization: Cytoplasm, Cell Surface and Secreted.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purification: Protein A/G Chromatography
• Storage: Store undiluted at 2-8°C.