Human IgA (alpha chain) Goat Polyclonal Antibody

CAT#: AP00640BT-N

Human IgA (alpha chain) goat polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody
  based assays using streptavidin or avidin conjugates:
  ELISA: 1/60,000-1/600,000
  Western Blot: 1/2,000-1/10,000
  Immunohistochemistry: 1/1,000-1/5,000
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Human IgA (alpha chain)
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Human IgA and Human Serum. No reaction was observed against other Human heavy or light chain proteins.
• Formulation: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2, containg 10 mg/ml BSA (IgG and Protease free) and 0.01% (w/v) Sodium Azide.
  Label: Biotin
  State: Lyophilized purified IgG fraction
  Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
  Molar radio: 10-20 BAC molecules per Goat IgG molecule
• Reconstitution Method: Restore with 1.0 ml of deionized water
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography.
• Conjugation: Biotin
• Storage: Store vial at 2-8°C prior to restoration.
  For extended storage after reconstitution, mix product with glycerol to 50% final, then aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
  This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use.