Human Adiponectin ELISA Kit (96 wells)

CAT#: EA100013

Human Adiponectin ELISA Kit (96 wells)

Specifications

Product Data

• Format: 96-well strip plate
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hours
• Signal: Colorimetric
• Curve Range: 0-250ng/ml
• Sample Type: Cell culture supernatant, serum, plasma
• Sample Volume: 5 ul
• Specificity: Natural and recombinant Human adiponectin
• Sensitivity: 0.975ng/ml
• Reactivities: Human
• Cross Reactivity: N/A
• Interference: No significant interference observed with available related molecules.
• Components:
  • Antibody-Coated Wells (1 plate, 96 wells)
  • 250 ng/ml Adiponectin Standard (1.4 ml /vial)
  • Standard Diluent (10 ml/vial, 1 vial)
  • Sample Diluent (50 ml/bottle, 1 bottle)
  • Enzyme Conjugate Concentrate 100X (0.2ml/vial, 1 vial)
  • Enzyme Conjugate Diluent (13 ml/vial)
  • 20X Wash Buffer (50 ml/bottle, 1 bottle)
  • TMB Reagent (11 ml/bottle, 1 bottle)
  • Stop Solution (11 ml/bottle, 1 bottle)
• Background: Adiponectin, also known as Adipocyte Complement-Related Protein of 30kDa (ACRP30), is a secreted serum protein expressed exclusively in differentiated adipocytes. Studies indicated that decreased plasma adiponectin concentration is associated with obesity, insulin resistance, essential hypertension, inflammation and atherosclerosis, and acute myocardial infarction. On the other hand, increased adiponectin level leads to nephrotic syndrome.
• Gene Symbol: ADIPOQ