Human IL-4 ELISA Kit (48-well)

CAT#: EA100021

Human IL-4 ELISA Kit (48-well)

Product Images

Representative standard curve for IL-4 ELISA. IL-4 was diluted in serial two-fold steps in Sample Diluent.

Specifications

Product Data

• Format: 48-well strip plate
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hours
• Signal: Colorimetric
• Curve Range: 7.8-500pg/ml
• Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
• Sample Volume: 20 uL
• Specificity: Natural and recombinant Human IL-4 Ligand
• Sensitivity: 2pg/mL
• Reactivities: Human
• Interference: No significant interference observed with available related molecules.
• Components:
  • Aluminium pouches with a Microwell Plate coated with monoclonal antibody to human IL-4 (8x6)
  • 2 vials human IL-4 Standard lyophilized, 500 pg/ml upon reconstitution
  • 2 vials concentrated Biotin-Conjugate anti-human IL-4 polyclonal antibody
  • 2 vials Streptavidin-HRP solution
  • 1 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 3 pieces Adhesive Films
  • package insert
• Background:

  Interleukin 4 (IL-4) is a pleiotropic cytokine produced primarily by activated T lymphocytes, mast cells and basophils . The sequence of human IL-4 cDNA predicts a 153 amino acid (aa) residue precursor protein containing a 24 aa residue signal peptide that is cleaved to form the mature protein . At the amino acid sequence level, mature human IL-4 is approximately 50% identical to mouse IL-4 and there is no species cross-reactivity between the two proteins . Human IL-4 also shares approximately 30% amino acid sequence identity to human IL-13 and the two cytokines exhibit overlapping biological activities . The gene for IL-4 has been mapped to human chromosome 5q, in close proximity to the genes for IL-3, IL-5, IL-13 and GM-CSF .

  IL-4 has multiple immune response-modulating functions on a variety of cell types. It is an important regulator of isotype switching, inducing IgE production in B lymphocytes. It is an important modulator of the differentiation of precursor T helper cells to the TH2 subset that mediates humoral immunity and modulates antibody production. In addition, IL-4 has also been shown to have anti-tumor activity both in vivo and in vitro .

  The biological effects of IL-4 are mediated by specific cell surface receptor complexes. One type of functional IL-4 receptor complex consists of the IL-4-binding subunit (IL-4 R) and a second chain, designated the common c chain because it has also been identified as a component of the receptor complexes for IL-2, IL-7, IL-9 and IL-15 . A second type of functional IL-4 receptor complex, consisting of the IL-4 R and the more recently cloned IL-13 R , has also been proposed .

  Although IL-4 R does not bind IL-13 directly, it has been shown to complex with the low-affinity IL-13 R to form the functional high-affinity receptor complex for IL-13 . In addition to the membrane-bound form of IL-4 R, a naturally occurring soluble form of IL-4 R has been identified in human and mouse biological fluids and in mouse cell culture supernates . Soluble IL-4 R has been to shown to bind IL-4 with high affinity in solution.

• Gene Symbol: IL4
• Standard Curve:
  
  Representative standard curve for IL-4 ELISA. IL-4 was diluted in serial two-fold steps in Sample Diluent.